Services

Our Core In Vitro Services

1. MTT Cytotoxicity Assay

Determine the anti-proliferative or cytotoxic potential of your sample using the gold-standard MTT assay.

• Cell Lines Available: MCF-7, HepG2, A549, HaCaT, HCT116, Vero, MRC-5, etc.
• Sample Types: Plant extracts, synthetic compounds, essential oils, nanoparticles.
• Output: IC₅₀ calculation, viability %, triplicate raw OD values, and SD.

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2. Scratch Assay (Wound Healing)

Assess cell migration and wound closure capacity of your test items.

• Used For: Natural wound healing products, growth factor studies, antimicrobial gels.
• Observation Points: 0 h, 12 h, 24 h, and 48 h
• Output: % wound closure, triplicate data, image analysis report.

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3. Antioxidant Assays

Evaluate the free radical scavenging capacity of your extracts or molecules.

• DPPH Assay
• ABTS Assay
• FRAP (on request)
• Results delivered as % inhibition, IC₅₀ values, and standard graphs.

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4. Dose-Response and IC₅₀ Profiling

We conduct detailed dose-response studies using 3 to 8 concentrations across multiple replicates.

• Analysis Tools: Linear/Non-linear regression, R² values, statistical validation.
• Use Case: Early-stage drug discovery, phytochemical screening, cytotoxic validation.

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5. Morphological and Microscopy-Based Observations

Visual documentation of treated and control cells under inverted or phase-contrast microscope.

• High-resolution photomicrographs
• Pre/post-treatment comparison
• Cellular integrity, rounding, detachment reports

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6. Customized Cell Line Screening

Don’t see your required cell line listed? We can procure and screen based on request.

• Custom protocol design
• Long-term collaborations for labs and institutions
• Human, animal, and cancer cell line availability

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